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A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

Type

Journal article

Journal

J Biol Chem

Publication Date

25/02/2000

Volume

275

Pages

5941 - 5946

Keywords

Adenosine Triphosphatases, Caffeine, DNA Helicases, DNA Repair, Fungal Proteins, Genetic Complementation Test, Models, Biological, Mutation, Phosphodiesterase Inhibitors, Plasmids, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, Temperature, Transcription Factor TFIID, Transcription Factor TFIIH, Transcription Factors, Transcription Factors, TFII, Two-Hybrid System Techniques, Ultraviolet Rays