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Forty-nine isolates of Burkholderia pseudomallei from sporadic cases of melioidosis in Malaysia over the past 18 years were examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of total deoxyribonucleic acid (DNA). Twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. Five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. PFGE revealed a number of different strains within these ribotypes, but some pairs of isolates from unrelated cases gave closely similar DNA profiles. These results are in agreement with Australian studies which showed a high prevalence of a few ribotypes of B. pseudomallei which are further divisible by genotyping, in areas where melioidosis is endemic.

Original publication




Journal article


Trans R Soc Trop Med Hyg

Publication Date





358 - 360


Adult, Aged, Burkholderia pseudomallei, DNA, Bacterial, DNA, Ribosomal, Deoxyribonuclease BamHI, Electrophoresis, Gel, Pulsed-Field, Female, Humans, Malaysia, Male, Melioidosis, Middle Aged, Restriction Mapping