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Mass spectrometry is often used to ascertain the accurate mass of purified protein samples prior to crystallization screening. However, in many cases data regarding the form of the protein crystallizing can also be useful, as this may differ from the original sample. Development of a simple method for the preparation and mass spectrometry of crystal-derived protein samples is described. The method is exemplified by the determination of the phosphorylation state of protein in a crystal derived from a mixture of phosphorylated and unphosphorylated protein.

Original publication

DOI

10.1107/S0907444905007262

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

05/2005

Volume

61

Pages

643 - 645

Keywords

Chromatography, High Pressure Liquid, Crystallization, Mass Spectrometry, Muramidase, Phosphorylation, Proteins, Spectrometry, Mass, Electrospray Ionization