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Extracting DNA from formalin-fixed and paraffin-embedded (FFPE) tissue remains a challenge, despite numerous attempts to develop a more effective method. Polymerase chain reaction (PCR) success rates with DNA extracted using current methods remain low. We extracted DNA from 140 long-term archived FFPE samples using a simple but effective deparaffinization method, removing the wax with mineral oil, and a commercially available DNA extraction kit. DNA quality was subsequently tested in a genotyping experiment with 14 microsatellite markers. High-quality DNA was obtained with a mean PCR success rate of 97% (range: 88-100%) across markers. The results suggested that DNA extracted using this novel method is likely to be suitable for genetic studies involving DNA fragments <200 bp.

Original publication

DOI

10.1016/j.ab.2009.08.016

Type

Journal article

Journal

Anal Biochem

Publication Date

15/12/2009

Volume

395

Pages

265 - 267

Keywords

DNA, Fixatives, Formaldehyde, Mineral Oil, Paraffin Embedding, Polymerase Chain Reaction, Time Factors