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Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.

Original publication

DOI

10.1007/s00294-004-0498-4

Type

Journal article

Journal

Curr Genet

Publication Date

06/2004

Volume

45

Pages

371 - 377

Keywords

Alkyl and Aryl Transferases, Base Sequence, Chromosomes, Cloning, Molecular, Diphosphates, Escherichia coli, Fungal Proteins, Gene Expression Regulation, Fungal, Genetic Complementation Test, Mucor, Polyisoprenyl Phosphates, Sequence Analysis, DNA