Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

A new structural gene, carG, involved in the biosynthesis of carotenoids in the fungus Mucor circinelloides was isolated by heterologous hybridisation, using a probe derived from the Gibberella fujikuroi ggs1 gene. Functional analyses in Escherichia coli showed that the encoded protein has geranylgeranyl pyrophosphate (GGPP) synthase activity. A comparison of the deduced protein with other GGPP synthases suggested that the carG gene might have evolved from other larger genes present in some fungi. The analysis of carG mRNA accumulation after blue light irradiation showed that the expression of this gene is up-regulated by blue light, as happens with the other structural genes involved in carotenogenesis in M. circinelloides. Analysis of the promoter region revealed the presence of several APE-like sequences, which participate in the blue-light regulation of the expression of different fungal genes. These sequences are also present in the above-mentioned Mucor genes and strongly support the idea that this gene plays an important role in the regulation of carotenoid synthesis, despite belonging to a more general metabolic pathway.

Original publication




Journal article


Current genetics

Publication Date





112 - 120


Area de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Avenida del Campo Charro s/n, 37007 Salamanca, Spain.


Mucor, Carotenoids, Alkyl and Aryl Transferases, Dimethylallyltranstransferase, DNA Primers, Blotting, Northern, Blotting, Southern, Genetic Complementation Test, Nucleic Acid Hybridization, Sequence Alignment, Sequence Analysis, DNA, Gene Expression Regulation, Fungal, Up-Regulation, Base Sequence, Plasmids, Light, Molecular Sequence Data, Farnesyltranstransferase