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Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.

Type

Journal article

Journal

J Biol Chem

Publication Date

04/11/1994

Volume

269

Pages

27549 - 27553

Keywords

Base Sequence, Cell Survival, DNA Primers, DNA, Fungal, Fungal Proteins, Gene Expression, Genes, Fungal, Humans, Molecular Sequence Data, RNA, Fungal, RNA, Messenger, Recombinant Proteins, Saccharomyces cerevisiae, Sequence Alignment, Sequence Homology, Amino Acid, Transcription Factors, Transcription Factors, TFII