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How transcription factors interpret the output from signal transduction pathways to drive distinct programs of gene expression is a key issue that underpins development and disease. The ubiquitously expressed basic-helix-loop-helix leucine zipper upstream stimulating factor-1 binds E-box regulatory elements (CANNTG) to regulate a wide number of gene networks. In particular, USF-1 is a key component of the tanning process. Following UV irradiation, USF-1 is phosphorylated by the p38 stress-activated kinase on threonine 153 and directly up-regulates expression of the POMC, MC1R, TYR, TYRP-1 and DCT genes. However, how phosphorylation on Thr-153 might affect the activity of USF-1 is unclear. Here we show that, in response to DNA damage, oxidative stress and cellular infection USF-1 is acetylated in a phospho-Thr-153-dependent fashion. Phospho-acetylated USF-1 is nuclear and interacts with DNA but displays altered gene regulatory properties. Phospho-acetylated USF-1 is thus proposed to be associated with loss of transcriptional activation properties toward several target genes implicated in pigmentation process and cell cycle regulation. The identification of this critical stress-dependent USF-1 modification gives new insights into understanding USF-1 gene expression modulation associated with cancer development.

Original publication

DOI

10.1074/jbc.M808605200

Type

Journal article

Journal

J Biol Chem

Publication Date

10/07/2009

Volume

284

Pages

18851 - 18862

Keywords

Acetylation, Amino Acid Sequence, Animals, DNA Damage, Gene Expression Regulation, Humans, Melanoma, Experimental, Mice, Molecular Sequence Data, Oxidative Stress, Phosphorylation, Protein Processing, Post-Translational, Threonine, Upstream Stimulatory Factors, p38 Mitogen-Activated Protein Kinases