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Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight.

Original publication




Journal article


Eur J Immunol

Publication Date





205 - 210


Animals, Antibodies, Antibody Specificity, Antigens, Binding Sites, Chemical Precipitation, Clone Cells, Electrophoresis, Polyacrylamide Gel, Female, Humans, Hybrid Cells, Hybridization, Genetic, Immunoenzyme Techniques, Mice, Mice, Inbred BALB C, Myeloma Proteins, T-Lymphocytes