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Here we report on a monoclonal antibody (IST-9) which distinguishes between human cellular and plasma fibronectin. Using beta-galactosidase-fibronectin fusion proteins expressed in E. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ED) which can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two other monoclonal antibodies (IST-1 and IST-2), specific for the heparin-binding domain 5 of fibronectin.

Type

Journal article

Journal

FEBS Lett

Publication Date

11/05/1987

Volume

215

Pages

269 - 273

Keywords

Antibodies, Monoclonal, Epitopes, Escherichia coli, Fibronectins, Humans, RNA Splicing, Recombinant Fusion Proteins, Recombinant Proteins, beta-Galactosidase