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Electron cryo tomography (cryoET) is an ideal technique to study virus-host interactions at molecular resolution. Imaging of biological specimens in a frozen-hydrated state assures a close to native environment. Various virus-host cell interactions have been analysed in this way, with the herpesvirus 'life' cycle being the most comprehensively studied. The data obtained were further integrated with fluorescence and soft X-ray cryo microscopy data applied on experimental systems covering a wide range of biological complexity. This hybrid approach combines dynamic with static imaging and spans a resolution range from micrometres to angstroms. Along selected aspects of the herpesvirus replication cycle, we describe dedicated combinations of approaches and how subsequent data integration enables insights towards a functional understanding of the underlying processes.

Original publication

DOI

10.1016/j.coviro.2014.01.008

Type

Journal article

Journal

Curr Opin Virol

Publication Date

04/2014

Volume

5

Pages

42 - 49

Keywords

Animals, Cryoelectron Microscopy, Electron Microscope Tomography, Herpesviridae, Herpesviridae Infections, Humans