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Little is known as to how cells ensure that organelle size and number are coordinated to correctly couple organelle biogenesis to the demands of proliferation or differentiation. OA1 is a melanosome-associated G-protein-coupled receptor involved in melanosome biogenesis during melanocyte differentiation. Cells lacking OA1 contain fewer, but larger, mature melanosomes. Here, we show that OA1 loss of function reduces both the basal expression and the α-melanocyte-stimulating hormone/cAMP-dependent induction of the microphthalmia-associated transcription factor (MITF), the master regulator of melanocyte differentiation. In turn, this leads to a significant reduction in expression of PMEL, a major melanosomal structural protein, but does not affect tyrosinase and melanin levels. In line with its pivotal role in sensing melanosome maturation, OA1 expression rescues melanosome biogenesis, activates MITF expression and thereby coordinates melanosome size and number, providing a quality control mechanism for the organelle in which resides. Thus, resident sensor receptors can activate a transcriptional cascade to specifically promote organelle biogenesis.

Original publication

DOI

10.1111/pcmr.12239

Type

Journal article

Journal

Pigment Cell Melanoma Res

Publication Date

07/2014

Volume

27

Pages

565 - 579

Keywords

Ocular Albinism type 1, melanosome biogenesis, microphthalmia-associated transcription factor, Animals, Base Sequence, Cell Differentiation, Cell Line, Eye Proteins, Gene Expression Regulation, Humans, Melanocytes, Melanosomes, Membrane Glycoproteins, Mice, Mice, Knockout, Microphthalmia-Associated Transcription Factor, Molecular Sequence Data, Receptors, G-Protein-Coupled, alpha-MSH, gp100 Melanoma Antigen