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In-Fusion™ cloning is a fl exible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. © Springer Science+Business Media New York 2014.

Original publication

DOI

10.1007/978-1-62703-764-8_15

Type

Journal article

Journal

Methods in Molecular Biology

Publication Date

01/01/2014

Volume

1116

Pages

209 - 234