Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Polymerases are essential for life, being responsible for replication, transcription, and the repair of nucleic acid molecules. Those that share a right-hand-shaped fold and catalytic site structurally similar to the DNA polymerase I of Escherichia coli may catalyze RNA- or DNA-dependent RNA polymerization, reverse transcription, or DNA replication in eukarya, archaea, bacteria, and their viruses. We have applied novel computational methods for structure-based clustering and phylogenetic analyses of this functionally diverse polymerase superfamily, which currently comprises six families. We identified a structural core common to all right-handed polymerases, composed of 57 amino acid residues, harboring two positionally and chemically conserved residues, the catalytic aspartates. The structural conservation within each of the six families is considerable, for example, the structural core shared by family Y DNA polymerases covers over 90% of the polymerase domain of the Sulfolobus solfataricus Dpo4. Our phylogenetic analyses propose an early separation of RNA-dependent polymerases that use primers from those that are primer-independent. Furthermore, the exchange of polymerase genes between viruses and their hosts is evident. Because of this horizontal gene transfer, the phylogeny of polymerases does not always reflect the evolutionary history of the corresponding organisms.

Original publication

DOI

10.1093/molbev/msu219

Type

Journal article

Journal

Mol Biol Evol

Publication Date

10/2014

Volume

31

Pages

2741 - 2752

Keywords

nucleic acid polymerase, protein evolution, structural alignment, structural classification, Amino Acid Sequence, Aspartic Acid, Automation, Laboratory, Bacteria, Bacterial Proteins, Catalytic Domain, Computational Biology, Conserved Sequence, DNA-Directed DNA Polymerase, Evolution, Molecular, Gene Transfer, Horizontal, Models, Molecular, Phylogeny, Viral Proteins, Viruses