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Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.

Original publication

DOI

10.1007/978-1-4939-2205-5_11

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2015

Volume

1258

Pages

209 - 222

Keywords

Animals, Escherichia coli, HEK293 Cells, Humans, Mammals, Recombinant Proteins, Transfection