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Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

Original publication

DOI

10.1016/j.bdq.2014.11.003

Type

Journal article

Journal

Biomol Detect Quantif

Publication Date

12/2014

Volume

2

Pages

1 - 3

Keywords

Quantitative, Real-time PCR, Standards, Streptococcus pneumoniae