Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.

Original publication

DOI

10.1093/nar/gkv373

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

26/05/2015

Volume

43

Pages

5221 - 5235

Keywords

Adenosine Diphosphate, Crystallography, X-Ray, DNA, Models, Molecular, Mutation, Protein Conformation, Protein Structure, Tertiary, RecQ Helicases, Single-Domain Antibodies, Zinc