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The increasing demand for production and characterization of diverse groups of recombinant proteins necessitates the analysis of several constructs and fusion tags in a variety of expression systems. The challenge is to screen multiple clones quickly for the desired properties. When using a eukaryotic system, such as baculovirus-mediated expression in insect cells, the total time required and the volume of culture needed to obtain reasonable results are limiting factors. This chapter focuses on addressing these issues by describing rapid small-scale expression as a mode of screening. The method allows the rapid identification of the best clone before scaling-up and the production of heter-ologous protein. © 2008 Humana Press.

Original publication

DOI

10.1007/978-1-59745-188-8_19

Type

Journal article

Journal

Methods in Molecular Biology

Publication Date

01/01/2008

Volume

439

Pages

269 - 289