Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

BACKGROUND: We previously reported that the target genes in sporadic mismatch repair (MMR)-deficient colorectal carcinomas (CRCs) in the distal colon differ from those occurring elsewhere in the colon. This study aimed to compare the target gene mutational pattern in microsatellite instability (MSI) CRC from Lynch syndrome patients stratified by tumour location and germline mutation, as well as with that of sporadic disease. METHODS: A series of CRC from Lynch syndrome patients was analysed for MSI in genes predicted to be selective MSI targets and known to be involved in several pathways of colorectal carcinogenesis. RESULTS: The most frequently mutated genes belong to the TGF-β superfamily pathway, namely ACVR2A and TGFBR2. A significantly higher frequency of target gene mutations was observed in CRC from patients with germline mutations in MLH1 or MSH2 when compared with MSH6. Mutations in microsatellite sequences (A)7 of BMPR2 and (A)8 of MSH3 were significantly more frequent in the distal CRC. Additionally, we observed differences in MSH3 and TGFBR2 mutational frequency between Lynch syndrome and sporadic MSI CRC regarding tumour location. CONCLUSIONS: Our results indicate that the pattern of genetic changes differs in CRC depending on tumour location and between Lynch syndrome and sporadic MSI CRC, suggesting that carcinogenesis can occur by different pathways even if driven by generalised MSI.

Original publication

DOI

10.1038/bjc.2015.281

Type

Journal article

Journal

Br J Cancer

Publication Date

11/08/2015

Volume

113

Pages

686 - 692

Keywords

Activin Receptors, Type II, Adaptor Proteins, Signal Transducing, Adolescent, Adult, Aged, Bone Morphogenetic Protein Receptors, Type II, Carcinogenesis, Colorectal Neoplasms, Colorectal Neoplasms, Hereditary Nonpolyposis, DNA-Binding Proteins, Female, Germ-Line Mutation, Humans, Male, Microsatellite Instability, Middle Aged, MutL Protein Homolog 1, MutS Homolog 2 Protein, MutS Homolog 3 Protein, Nuclear Proteins, Protein-Serine-Threonine Kinases, Receptors, Transforming Growth Factor beta, Young Adult