High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.

Original publication

DOI

10.1038/nchembio.1933

Type

Journal article

Journal

Nat Chem Biol

Publication Date

12/2015

Volume

11

Pages

952 - 954

Keywords

HEK293 Cells, High-Throughput Screening Assays, Humans, Light, Protein Kinase Inhibitors, Protein Kinases, Small Molecule Libraries, Structure-Activity Relationship