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Memory B cells are long-lived and could contribute to persistence of humoral immunity by maintaining the plasma-cell pool or making recall responses upon re-exposure to an antigen. We determined the ability of a pneumococcal conjugate vaccine to induce anti-pneumococcal memory B cells. Frequencies of memory B cells against pneumococcal capsular polysaccharides from serotypes 1, 6B, 14, 19F and 23F were determined by cultured B cell enzyme-linked immunospot (ELISPOT) in 35 children aged 12-23 months who received pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). The relationships between plasma antibodies and memory B cell frequencies were also assessed. After two doses of PHiD-CV, the proportion of subjects with detectable memory B cells against pneumococcal capsular polysaccharides increased significantly for serotypes 1 (3-45%; P < 0·01), 19F (21-66%; P < 0·01) and 23F (13-36%; P = 0·02), but not serotypes 6B (24-42%; P = 0·24) and 14 (21-40%; P = 0·06). Correlations between antibodies and memory B cells were weak. Carriage of serotype 19F at enrolment was associated with poor memory B cell responses against this serotype at subsequent time-points (day 30: non-carriers, 82% versus carriers, 0%, P < 0·01; day 210: non-carriers, 72% versus carriers, 33%, P = 0·07). PHiD-CV is capable of inducing memory B cells against some of the component pneumococcal capsular polysaccharides.

Original publication

DOI

10.1111/cei.12637

Type

Journal article

Journal

Clin Exp Immunol

Publication Date

08/2015

Volume

181

Pages

297 - 305

Keywords

ELISPOT, PHiD-CV, children, memory B cells, pneumococcal conjugate vaccines, Antibodies, Bacterial, B-Lymphocytes, Bacterial Proteins, Carrier Proteins, Female, Haemophilus Infections, Haemophilus Vaccines, Haemophilus influenzae, Humans, Immunity, Humoral, Immunoglobulin D, Immunologic Memory, Infant, Kenya, Lipoproteins, Male, Polysaccharides, Bacterial, Serotyping, Treatment Outcome, Vaccination, Vaccines, Conjugate