Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity.
Hamed RB., Gomez-Castellanos JR., Sean Froese D., Krysztofinska E., Yue WW., Schofield CJ.
The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.