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We recently developed a new assay to measure proteasome activity in vitro (CAPA for capture proteasome assay) [1], based on proteasome capture on an antibody-coated plate. When used with lysates originating from cells expressing either standard proteasome, immunoproteasome or intermediate proteasomes β5i or β1i-β5i, this assay allows the individual monitoring of the chymotrypsin-like, trypsin-like and caspase-like activities of the corresponding proteasome subtypes. The efficiency and specificity of four proteasome inhibitors were studied using the CAPA assay, demonstrating the potential of this assay for the development of subtype-specific proteasome inhibitors.

Original publication

DOI

10.1016/j.dib.2015.04.019

Type

Journal article

Journal

Data Brief

Publication Date

09/2015

Volume

4

Pages

146 - 151

Keywords

Activity assay, Fluorogenic peptides, Proteasome, Proteasome-Glo