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Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.

Original publication

DOI

10.1007/978-1-62703-218-6_16

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2013

Volume

960

Pages

187 - 207

Keywords

Animals, Antigen Presentation, COS Cells, Cell Line, Cercopithecus aethiops, Escherichia coli, Gene Library, HEK293 Cells, Histocompatibility Antigens Class I, Humans, Peptide Fragments, Phosphorylation, T-Lymphocytes, Cytotoxic, Transfection, Transformation, Genetic, Tumor Necrosis Factor-alpha