CD8-positive T lymphocytes recognize peptides that are usually derived from the degradation of cellular proteins and are presented by class I molecules of the major histocompatibility complex. Here we describe a human minor histocompatibility antigen created by a polymorphism in the SP110 nuclear phosphoprotein gene. The antigenic peptide comprises two noncontiguous SP110 peptide segments spliced together in reverse order to that in which they occur in the predicted SP110 protein. The antigenic peptide could be produced in vitro by incubation of precursor peptides with highly purified 20S proteasomes. Cutting and splicing probably occur within the proteasome by transpeptidation.
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Alleles, Amino Acid Motifs, Amino Acid Sequence, Amino Acid Substitution, Antigen Presentation, B-Lymphocytes, Cell Line, Transformed, Cytotoxicity, Immunologic, Electroporation, HLA-A Antigens, Humans, Interferon-gamma, Male, Middle Aged, Minor Histocompatibility Antigens, Molecular Sequence Data, Nuclear Proteins, Peptide Fragments, Polymorphism, Single Nucleotide, Proteasome Endopeptidase Complex, Protein Splicing, T-Lymphocytes, Cytotoxic