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Disruptions to neuronal mRNA translation are hypothesized to underlie human neurodevelopmental syndromes. Notably, the mRNA translation re-initiation factor DENR is a regulator of eukaryotic translation and cell growth, but its mammalian functions are unknown. Here, we report that Denr influences the migration of murine cerebral cortical neurons in vivo with its binding partner Mcts1, whereas perturbations to Denr impair the long-term positioning, dendritic arborization, and dendritic spine characteristics of postnatal projection neurons. We characterized de novo missense mutations in DENR (p.C37Y and p.P121L) detected in two unrelated human subjects diagnosed with brain developmental disorder to find that each variant impairs the function of DENR in mRNA translation re-initiation and disrupts the migration and terminal branching of cortical neurons in different ways. Thus, our findings link human brain disorders to impaired mRNA translation re-initiation through perturbations in DENR (OMIM: 604550) function in neurons.

Original publication

DOI

10.1016/j.celrep.2016.04.090

Type

Journal article

Journal

Cell Rep

Publication Date

07/06/2016

Volume

15

Pages

2251 - 2265

Keywords

Animals, Cell Differentiation, Cell Movement, Cerebral Cortex, Eukaryotic Initiation Factors, Gene Knockdown Techniques, Humans, Mice, Inbred C57BL, Mutant Proteins, Mutation, Nervous System Diseases, Neurogenesis, Neurons, Peptide Chain Initiation, Translational, RNA, Messenger