Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA base in mammalian cells that is believed to be another important epigenetic modification. Here we report the use of a methylation-insensitive restriction enzyme Taq(α)I coupled with selective chemical labeling of 5-hmC in a combined glycosylation restriction analysis (CGRA) to detect 5-hmC in TCGA sequences. This method, differentiates fully versus hemi-hydroxymethylated cytosine in the CpG dinucleotide, adds a new tool to facilitate biological studies of 5-hmC.

Original publication

DOI

10.1016/j.bmcl.2011.03.118

Type

Journal article

Journal

Bioorg Med Chem Lett

Publication Date

01/09/2011

Volume

21

Pages

5075 - 5077

Keywords

5-Methylcytosine, Base Sequence, Cytosine, DNA, DNA-Directed RNA Polymerases, Glycosylation