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In model organisms, classical genetic screening via random mutagenesis provides key insights into the molecular bases of genetic interactions, helping to define synthetic lethality, synthetic viability and drug-resistance mechanisms. The limited genetic tractability of diploid mammalian cells, however, precludes this approach. Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.

Original publication

DOI

10.1038/nchembio.2226

Type

Journal article

Journal

Nat Chem Biol

Publication Date

01/2017

Volume

13

Pages

12 - 14

Keywords

Animals, Cell Line, Genetic Testing, Genome, Mice, Mouse Embryonic Stem Cells, Mutagenesis