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HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.

Original publication

DOI

10.1073/pnas.0832180100

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

10/06/2003

Volume

100

Pages

7271 - 7276

Keywords

Antibodies, Monoclonal, Antigens, CD, Cell Line, Chimera, HIV, HeLa Cells, Hepacivirus, Hepatitis C Antibodies, Hepatocytes, Humans, Hydrogen-Ion Concentration, Membrane Proteins, Neutralization Tests, Receptors, Virus, Tetraspanin 28, Viral Envelope Proteins