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A small 14 amino acid oligopeptide tag (termed SV5-Pk) was fused onto the carboxy-terminus of simian immunodeficiency virus gp160 expressed from a recombinant baculovirus. The presence of the Pk tag had no obvious effect on the expression and glycosylation of gp160 and did not interfere either with CD4 binding or with cleavage at its maturation site by the protease furin. The presence of the Pk tag did, however, facilitate the simplified purification of full-length gp160 and its incorporation into immunogenic solid matrix-antibody-antigen (SMAA) complexes.

Type

Journal article

Journal

J Virol Methods

Publication Date

05/1995

Volume

53

Pages

149 - 156

Keywords

Animals, Antigen-Antibody Complex, Baculoviridae, CD4 Antigens, Epitopes, Gene Products, env, Oligopeptides, Protein Binding, Protein Processing, Post-Translational, Recombinant Fusion Proteins