Central Nervous System Infection Diagnosis by Next-Generation Sequencing: A Glimpse Into the Future?
Mai NTH., Phu NH., Nhu LNT., Hong NTT., Hanh NHH., Nguyet LA., Phuong TM., McBride A., Ha DQ., Nghia HDT., Chau NVV., Thwaites G., Tan LV.
Japanese encephalitis virus was detected by deep sequencing for the first time in urine of a 16-year-old boy with encephalitis. Seroconversion and polymerase chain reaction analysis confirmed the metagenomics finding. Urine is useful for diagnosis of flaviviral encephalitis, whereas deep sequencing can be a panpathogen assay for the diagnosis of life-threatening infectious diseases.