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We used the vapor phase of acrolein as an anhydrous fixative for timothy grass pollen in an immunogold double-labeling localization study of two different major allergens, Phl p I and Phl p V. More than 48 hr of fixation were needed for the subcellular pollen structures to be satisfactorily stabilized. The immunoreactivity of acrolein-fixed pollen allergens was not destroyed even after prolonged acrolein fixation. By immunoblotting, the two allergens differ in their immunological and structural characteristics. Electron microscopic localization traced the allergens at least partially to different subcellular pollen compartments.

Original publication

DOI

10.1177/42.3.8308259

Type

Journal article

Journal

J Histochem Cytochem

Publication Date

03/1994

Volume

42

Pages

427 - 431

Keywords

Acrolein, Allergens, Animals, Antibodies, Monoclonal, Fixatives, Gases, Immunoblotting, Immunohistochemistry, Mice, Microscopy, Immunoelectron, Poaceae, Pollen, Rabbits, Tissue Fixation