A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
Siridechadilok B., Gomutsukhavadee M., Sawaengpol T., Sangiambut S., Puttikhunt C., Chin-inmanu K., Suriyaphol P., Malasit P., Screaton G., Mongkolsapaya J.
<jats:p>Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.</jats:p>