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We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues.

Original publication

DOI

10.1016/j.jsb.2017.10.010

Type

Journal article

Journal

J Struct Biol

Publication Date

04/2018

Volume

202

Pages

94 - 99

Keywords

Cryo electron microscopy, Density modification, Model building, Virus structure