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Sexually mature male and female Brugia malayi were developed from third stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop Med Hyg 37: 370-375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200-1,500 microfilariae/day.


Journal article


Am J Trop Med Hyg

Publication Date





3 - 5


Animals, Brugia, Culture Media, Female, Fertility, Humans, Male