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Objective: To observe the effect of the iASPP RNAi vector transfection on the apoptosis of breat cancer cell MCF-7. Methods: The specific siRNA sequence was designed according to the genebank. The sequence was cloned into PGCsilencer™ H1/Neo/GFP. The recombinant plasmid was transfected into MCF-7 by Lipofectamine™ 2000. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. Results: The iASPP expression in MCF-7 cell lines was descended from 40% to 50% after the RNAi transfection. The expression of p53 protein increased from 0.37 to 0.64, and the apoptosis rate increased from 17.8% and 16.2% to 53.5% and 51.3%. Conclusion: The inhibition of endogenous iASPP may resume the apoptosis activity of p53 in MCF-7.

Type

Journal article

Journal

Chinese Journal of Cancer Prevention and Treatment

Publication Date

01/11/2008

Volume

15