Interleukin-22 promotes phagolysosomal fusion to induce protection againstSalmonella entericaTyphimurium in human epithelial cells
Forbester JL., Lees EA., Goulding D., Forrest S., Yeung A., Speak A., Clare S., Coomber EL., Mukhopadhyay S., Kraiczy J., Schreiber F., Lawley TD., Hancock REW., Uhlig HH., Zilbauer M., Powrie F., Dougan G.
Intestinal epithelial cells (IECs) play a key role in regulating immune responses and controlling infection. However, the direct role of IECs in restricting pathogens remains incompletely understood. Here, we provide evidence that IL-22 primed intestinal organoids derived from healthy human induced pluripotent stem cells (hIPSCs) to restrictSalmonella entericaserovar Typhimurium SL1344 infection. A combination of transcriptomics, bacterial invasion assays, and imaging suggests that IL-22–induced antimicrobial activity is driven by increased phagolysosomal fusion in IL-22–pretreated cells. The antimicrobial phenotype was absent in hIPSCs derived from a patient harboring a homozygous mutation in theIL10RBgene that inactivates the IL-22 receptor but was restored by genetically complementing the IL10RB deficiency. This study highlights a mechanism through which the IL-22 pathway facilitates the human intestinal epithelium to control microbial infection.