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While all basic region/helix-loop-helix (bHLH) proteins bind the consensus CANNTG motif, other factors must be involved in determining regulatory specificity. In this report we show that bases outside this core 6 bp are involved in determining the specificity of binding. Thus, binding of the yeast bHLH protein PHO4, but not CPF-1, is inhibited by the presence of a T residue immediately 5' to their common CACGTG recognition sequence. PHO4 binding specificity is altered by mutation at any of three different positions in the basic region, including a single Glu to Asp substitution. The significance of these data for DNA-binding and transcription regulation by the bHLH family of transcription factors is discussed.

Type

Journal article

Journal

EMBO J

Publication Date

11/1992

Volume

11

Pages

4103 - 4109

Keywords

Amino Acid Sequence, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Binding Sites, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Methionine, Molecular Sequence Data, Mutagenesis, Site-Directed, Oligonucleotide Probes, Phosphoric Monoester Hydrolases, Protein Conformation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Nucleic Acid, Transcription Factors