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The basic-helix-loop-helix (b-HLH) motif is common to a number of proteins involved in transcriptional regulation and cell-type determination. The b-HLH motif is also present in the S. cerevisiae transcription factor PHO4 which positively regulates the acid phosphatase gene PHO5. In this report we show that the b-HLH region of PHO4 is sufficient to confer specific DNA-binding to the sequence CACGTG and, by comparison of the basic regions of PHO4 with those of other recently isolated CACGTG-binding proteins, we identify a specific subset of conserved amino acids in the basic region likely to confer DNA-binding specificity. On the basis of these observations we predict successfully the effect of substituting the PHO4 basic region with that from c-myc and show that the chimaeric protein activates transcription from the CACGTG elements present in the PHO5 UAS. From these data it is clear that the myc basic region confers specific binding to the sequence CACGTG.

Type

Journal article

Journal

Oncogene

Publication Date

07/1991

Volume

6

Pages

1099 - 1104

Keywords

Amino Acid Sequence, Base Sequence, Binding Sites, DNA, DNA-Binding Proteins, Deoxyribonuclease I, Fungal Proteins, Molecular Sequence Data, Nucleic Acid Conformation, Proto-Oncogene Proteins c-myc, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Nucleic Acid, Transcription Factors, Transcription, Genetic