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The octamer-binding proteins present in HeLa cells, B-cells and malignant melanoma cells were compared by a gel-electrophoresis DNA-binding assay. Using an extract from the malignant melanoma cells a complex was formed using a variety of octamer containing probes that was distinct from those found using either a HeLa or B-cell extract. DNAase 1 footprints and methylation interference patterns of the melanoma-specific octamer-binding protein were indistinguishable from those obtained with the HeLa factor NF-A1, except for preferential binding of the melanoma-specific factor to DNA methylated at two G residues 16 base-pairs 3' to the octamer motif. Competition analyses using a variety of wild-type and mutant probes showed that mutations affecting binding of NF-A1 similarly affected binding of the melanoma octamer-binding factor. These data also revealed the extreme flexibility of the octamer-binding site, with one probe sharing only 4 bases with the 8 base consensus sequence binding efficiently.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

09/12/1988

Volume

16

Pages

11047 - 11056

Keywords

B-Lymphocytes, Base Composition, Cell Line, DNA Modification Methylases, DNA, Neoplasm, DNA-Binding Proteins, Deoxyribonuclease I, HeLa Cells, Humans, Melanoma, Oligonucleotide Probes, Sequence Homology, Nucleic Acid