Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the level of specific transcription in nuclei isolated from transfected cells we directly demonstrate that the increased amount of EIIa RNA is due to stimulation of the rate of transcription.

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

07/1984

Volume

81

Pages

4381 - 4385

Keywords

Adenoviridae, Gene Expression Regulation, HeLa Cells, Humans, Operon, Plasmids, RNA, Messenger, RNA, Viral, Recombination, Genetic, Transcription, Genetic, Transfection, Viral Proteins