Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Recent reports have described reduced populations of CD27+ memory B cells and increased percentages of undifferentiated B cells in peripheral blood of patients with common variable immunodeficiency (CVID). This work has prompted two attempts to classify CVID based on rapid flow cytometric quantification of peripheral blood memory B cells and immature B cells. Evidence to support the hypothesis that such in vitro B cell classification systems correlate with clinical subtypes of CVID is being sought. For the classification to be useful in routine diagnosis, it is important that the flow cytometric method can be used without prior separation of peripheral blood mononuclear cells (PBMC). We have examined 23 CVID patients and 24 controls, using both PBMC and whole blood, and find an excellent correlation between these methods. The reproducibility of the method was excellent. We classified the CVID patients by all three of the existing classifications, including secretion of immunoglobulin by B cells in vitro as described by Bryant, as well as the more recent flow cytometric classification methods. Only one patient changed classification as a result of using whole blood.

Original publication

DOI

10.1111/j.1365-2249.2005.02793.x

Type

Journal article

Journal

Clin Exp Immunol

Publication Date

06/2005

Volume

140

Pages

532 - 539

Keywords

Adolescent, Adult, Aged, B-Lymphocytes, Common Variable Immunodeficiency, Female, Flow Cytometry, Humans, Immunologic Memory, Leukocytes, Mononuclear, Lymphocyte Count, Male, Middle Aged, Receptors, Complement 3d, Reproducibility of Results, Tumor Necrosis Factor Receptor Superfamily, Member 7