Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate
Yuan F., Bi Y., Siejka-Zielinska P., Zhou Y-L., Zhang X-X., Song C-X.
WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm 5 C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m 5 C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).