Dr, Transgenic Core Head
Genetically modified models represent one of the most powerful methods of functional gene analysis in vivo. Furthermore, the ability to introduce specific mutations into the genome enables models of human disease to be generated, facilitating insights into the pathophysiology of disease and providing a model with which therapeutic strategies and diagnostic tools can be optimized.
Our group provides groups within Oxford University access to transgenic technologies both on a fee-for-service type arrangement and on a collaborative basis. Technologies offered include embryo microinjection, embryonic stem cell transfection, Knock-out/-in construct design and in vivo shRNA mediated gene Knock-down. In addition, embryo rederivation and cryoconservation services are offered to facilitate the management, transfer and security of genetically modified strains.
The research activity of the group is focused on the development of novel methodologies for the generation of genetically modified models. The aims being to improve the reliability of the technology and to reduce the animal cost of research involving genetically modified models.
|Fluorescent 1 and 2 cell embryos|
|A single ES cell colony expressing Green Fluorescent Protein growing on a fibroblast feeder layer|
|Microinjection of embryonic stem cells into a blastocyst|
When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping.
Sailer S. et al, (2021), Cell Biosci, 11
Phenotype of a transient neonatal diabetes point mutation (SUR1-R1183W) in mice
Sachse G. et al, (2021), Wellcome Open Research, 5, 15 - 15
Replacement of surgical vasectomy through the use of wild-type sterile hybrids
Preece C. et al, (2021), Lab Animal
The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.
Castroflorio E. et al, (2020), Cellular and molecular life sciences : CMLS
Fluorescent PSC-Derived Cardiomyocyte Reporter Lines: Generation Approaches and Their Applications in Cardiovascular Medicine
Sontayananon N. et al, (2020), Biology, 9, 402 - 402